6hn7

X-ray diffraction
3Å resolution

Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit.

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-105386 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Redirecting phage packaging protein C (RppC) Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 17.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A5H1ZR32 (Residues: 1-153; Coverage: 100%)
Gene name: CXXR01000010.1
Terminase small subunit Chain: B
Molecule details ›
Chain: B
Length: 101 amino acids
Theoretical weight: 11.47 KDa
Source organism: Lambdavirus lambda
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P03707 (Residues: 1-98; Coverage: 54%)
Gene names: Nu1, lambdap01
Sequence domains: Phage DNA packaging protein Nu1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: C2221
Unit cell:
a: 60.523Å b: 101.004Å c: 82.627Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.314 0.312 0.332
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'