› 6hsv

X-ray diffraction
2.45Å resolution

Engineered higher-order assembly of Cholera Toxin B subunits via the addition of C-terminal parallel coiled-coiled domains

Released:
DOI: 10.2210/pdb6hsv/pdb
Model geometry
Fit model/data
PDB entry 6hsv coloured by chain, front view.
PDB entry 6hsv coloured by chain, side view.
PDB entry 6hsv coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Directed Assembly of Homopentameric Cholera Toxin B-Subunit Proteins into Higher-Order Structures Using Coiled-Coil Appendages.
Ross JF, Wildsmith GC, Johnson M, Hurdiss DL, Hollingsworth K, Thompson RF, Mosayebi M, Trinh CH, Paci E, Pearson AR, Webb ME, Turnbull WB
OpenAccess logo J Am Chem Soc 141 5211-5219 (2019)
PMID: 30856321

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo 120-mer
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholera enterotoxin subunit B Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Length: 124 amino acids
Theoretical weight: 13.88 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01556 (Residues: 22-124; Coverage: 100%)
Gene names: VC_1456, ctxB, toxB
Sequence domains: Heat-labile enterotoxin beta chain

Ligands and Environments

3 bound ligands:
Ligand PO4 6 x PO4
Ligand MPD 27 x MPD
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P63
Unit cell:
a: 179.16Å b: 179.16Å c: 192.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.195 0.214
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

De Novo Designed Peptide and Protein Hairpins Self-Assemble into Sheets and Nanoparticles.
Galloway et al. (2021)
5 other articles

1 mention without citation

Directed Assembly of Homopentameric Cholera Toxin B-Subunit Proteins into Higher-Order Structures Using Coiled-Coil Appendages.
Ross et al. (2019)