› 6ht7

X-ray diffraction
3.7Å resolution

Crystal structure of the WT human mitochondrial chaperonin (ADP:BeF3)14 complex

Released:
DOI: 10.2210/pdb6ht7/pdb
PDB entry 6ht7 coloured by chain, front view.
PDB entry 6ht7 coloured by chain, side view.
PDB entry 6ht7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the WT human mitochondrial football Hsp60-Hsp10(ADPBeFx)14 complex
Jebara F, Patra M, Azem A
Nat Commun (2020)

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145457 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
60 kDa heat shock protein, mitochondrial Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 549 amino acids
Theoretical weight: 58.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10809 (Residues: 27-573; Coverage: 96%)
Gene names: HSP60, HSPD1
Sequence domains: TCP-1/cpn60 chaperonin family
10 kDa heat shock protein, mitochondrial Chains: 1, 2, O, P, Q, R, S, T, U, V, W, X, Y, Z
Molecule details ›
Chains: 1, 2, O, P, Q, R, S, T, U, V, W, X, Y, Z
Length: 102 amino acids
Theoretical weight: 10.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61604 (Residues: 1-102; Coverage: 100%)
Gene name: HSPE1
Sequence domains: Chaperonin 10 Kd subunit

Ligands and Environments

4 bound ligands:
Ligand ADP 14 x ADP
Ligand BEF 14 x BEF
Ligand MG 14 x MG
Ligand K 14 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 141.59Å b: 295.78Å c: 326.53Å
α: 90° β: 90° γ: 90°
Expression system: Escherichia coli

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