› 6huf

X-ray diffraction
2.82Å resolution

Coping with strong translational non-crystallographic symmetry and extreme anisotropy in molecular replacement with Phaser: human Rab27a

Released:
DOI: 10.2210/pdb6huf/pdb
PDB entry 6huf coloured by chain, front view.
PDB entry 6huf coloured by chain, side view.
PDB entry 6huf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Coping with strong translational noncrystallographic symmetry and extreme anisotropy in molecular replacement with Phaser: human Rab27a.
Jamshidiha M, Pérez-Dorado I, Murray JW, Tate EW, Cota E, Read RJ
Acta Crystallogr D Struct Biol 75 342-353 (2019)
PMID: 30950405

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-27A Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 182 amino acids
Theoretical weight: 20.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P51159 (Residues: 2-192; Coverage: 82%)
Gene names: RAB27, RAB27A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 16 x MG
Ligand GNP 16 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 130.42Å b: 132.41Å c: 230.59Å
α: 90° β: 103.57° γ: 90°
R-values:
R R work R free
0.313 0.312 0.342
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Introduction to molecular replacement: a time perspective.
Dodson E. (2021)
5 other articles

3 mentions without citation

Rab GTPases: Emerging Oncogenes and Tumor Suppressive Regulators for the Editing of Survival Pathways in Cancer.
Gopal Krishnan et al. (2020)
2 more