› 6hv9

Electron Microscopy
4.98Å resolution

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O = ADP + phosphate
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-219800 (preferred)
Entry contents:
13 distinct polypeptide molecules
3 distinct DNA molecules
Macromolecules (16 distinct):
DNA replication licensing factor MCM3 Chain: 3
Molecule details ›
Chain: 3
Length: 971 amino acids
Theoretical weight: 107.65 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P24279 (Residues: 1-971; Coverage: 100%)
Gene names: MCM3, SYGP-ORF23, YEL032W
Sequence domains:
DNA replication licensing factor MCM4 Chain: 4
Molecule details ›
Chain: 4
Length: 933 amino acids
Theoretical weight: 105.14 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P30665 (Residues: 1-933; Coverage: 100%)
Gene names: CDC54, HCD21, MCM4, YP9531.13, YPR019W
Sequence domains:
Minichromosome maintenance protein 5 Chain: 5
Molecule details ›
Chain: 5
Length: 775 amino acids
Theoretical weight: 86.51 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29496 (Residues: 1-775; Coverage: 100%)
Gene names: CDC46, L9328.1, MCM5, YLR274W
Sequence domains:
DNA replication licensing factor MCM6 Chain: 6
Molecule details ›
Chain: 6
Length: 1017 amino acids
Theoretical weight: 113.11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P53091 (Residues: 1-1017; Coverage: 100%)
Gene names: MCM6, YGL201C
Sequence domains:
DNA replication licensing factor MCM2 Chain: 2
Molecule details ›
Chain: 2
Length: 868 amino acids
Theoretical weight: 98.91 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29469 (Residues: 1-868; Coverage: 100%)
Gene names: MCM2, YBL023C, YBL0438
Sequence domains:
DNA replication licensing factor MCM7 Chain: 7
Molecule details ›
Chain: 7
Length: 845 amino acids
Theoretical weight: 95.05 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38132 (Residues: 1-845; Coverage: 100%)
Gene names: CDC47, MCM7, YBR1441, YBR202W
Sequence domains:
DNA replication complex GINS protein PSF1 Chain: C
Molecule details ›
Chain: C
Length: 208 amino acids
Theoretical weight: 24.23 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12488 (Residues: 1-208; Coverage: 100%)
Gene names: PSF1, PZA208, YD8119.18, YDR013W
Sequence domains: GINS complex protein
DNA replication complex GINS protein PSF2 Chain: D
Molecule details ›
Chain: D
Length: 213 amino acids
Theoretical weight: 25.1 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40359 (Residues: 1-213; Coverage: 100%)
Gene names: HRF213, J1086, PSF2, YJL072C
Sequence domains: GINS complex protein
DNA replication complex GINS protein PSF3 Chain: E
Molecule details ›
Chain: E
Length: 194 amino acids
Theoretical weight: 21.98 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12146 (Residues: 1-194; Coverage: 100%)
Gene names: PSF3, YOL146W
Sequence domains: GINS complex protein
DNA replication complex GINS protein SLD5 Chain: F
Molecule details ›
Chain: F
Length: 294 amino acids
Theoretical weight: 33.98 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03406 (Residues: 1-294; Coverage: 100%)
Gene names: SLD5, YDR489W
Sequence domains:
Cell division control protein 45 Chain: G
Molecule details ›
Chain: G
Length: 650 amino acids
Theoretical weight: 74.32 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q08032 (Residues: 1-650; Coverage: 100%)
Gene names: CDC45, L8004.11, SLD4, YLR103C
Sequence domains: CDC45-like protein
DNA polymerase epsilon subunit B Chain: B
Molecule details ›
Chain: B
Length: 689 amino acids
Theoretical weight: 78.41 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P24482 (Residues: 1-689; Coverage: 100%)
Gene names: DPB2, P9705.7, YPR175W
Sequence domains: DNA polymerase alpha/epsilon subunit B
DNA polymerase epsilon catalytic subunit A Chain: A
Molecule details ›
Chain: A
Length: 914 amino acids
Theoretical weight: 104.98 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P21951 (Residues: 1308-2221; Coverage: 41%)
Gene names: DUN2, N0825, POL2, YNL262W
Sequence domains: Domain of unknown function (DUF1744)
DNA (5'-D(*GP*CP*AP*GP*CP*CP*AP*CP*GP*CP*TP*GP*GP*CP*CP*GP*TP*TP*TP*TP*A)-3') Chain: X
Molecule details ›
Chain: X
Length: 21 nucleotides
Theoretical weight: 6.42 KDa
DNA (5'-D(P*TP*AP*AP*AP*AP*CP*GP*GP*CP*CP*AP*GP*CP*GP*TP*GP*GP*CP*TP*GP*C)-3') Chain: Y
Molecule details ›
Chain: Y
Length: 21 nucleotides
Theoretical weight: 6.47 KDa
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*T)-3') Chain: J
Molecule details ›
Chain: J
Length: 7 nucleotides
Theoretical weight: 2.08 KDa

Ligands and Environments

2 bound ligands:
Ligand AGS 2 x AGS
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.98Å
Relevant EMDB volumes: EMD-0288
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided

Quick links

Citations

11 review citations

Different mechanisms for translocation by monomeric and hexameric helicases.
Gao et al. (2020)
10 more

5 mentions without citation

Structure of a human replisome shows the organisation and interactions of a DNA replication machine.
Jones et al. (2021)
4 more