› 6hw1

X-ray diffraction
2.5Å resolution

ROOM TEMPERATURE STRUCTURE OF LIPASE FROM T. LANUGINOSA AT 2.5 A RESOLUTION IN CHIPX MICROFLUIDIC DEVICE

Released:
DOI: 10.2210/pdb6hw1/pdb
PDB entry 6hw1 coloured by chain, front view.
PDB entry 6hw1 coloured by chain, side view.
PDB entry 6hw1 coloured by chain, top view.
Source organism: Thermomyces lanuginosus
Primary publication:
A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography.
de Wijn R, Hennig O, Roche J, Engilberge S, Rollet K, Fernandez-Millan P, Brillet K, Betat H, Mörl M, Roussel A, Girard E, Mueller-Dieckmann C, Fox GC, Olieric V, Gavira JA, Lorber B, Sauter C
IUCrJ 6 454-464 (2019)
PMID: 31098026

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129891 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 29.34 KDa
Source organism: Thermomyces lanuginosus
UniProt:
  • Canonical: O59952 (Residues: 23-291; Coverage: 98%)
Gene name: LIP
Sequence domains:
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand NAG 2 x NAG
Ligand PO4 1 x PO4
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P61
Unit cell:
a: 142.652Å b: 142.652Å c: 80.721Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.172 0.199

Quick links

Citations

8 citation in other articles

Facile Fabrication of Protein-Macrocycle Frameworks.
Ramberg et al. (2021)
7 more

3 mentions without citation

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET.
Magalhães et al. (2021)
2 more