› 6hyi

X-ray diffraction
1.4Å resolution

Regulatory subunit of a cAMP-independent protein kinase A from Trypanosoma cruzi at 1.4 A resolution in complex with inosine

Released:
DOI: 10.2210/pdb6hyi/pdb
PDB entry 6hyi coloured by chain, front view.
PDB entry 6hyi coloured by chain, side view.
PDB entry 6hyi coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
Purine nucleosides replace cAMP in allosteric regulation of PKA in trypanosomatid pathogens.
Ober VT, Githure GB, Volpato Santos Y, Becker S, Moya Munoz G, Basquin J, Schwede F, Lorentzen E, Boshart M
Elife 12 (2024)
PMID: 38517938

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183143 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic nucleotide-binding domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 304 amino acids
Theoretical weight: 34.48 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q86RB0 (Residues: 200-503; Coverage: 60%)
Gene names: C3747_37g237, ECC02_004294, PKAR
Sequence domains: Cyclic nucleotide-binding domain

Ligands and Environments

1 bound ligand:
Ligand NOS 2 x NOS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 40.74Å b: 88.529Å c: 46.02Å
α: 90° β: 98.28° γ: 90°
R-values:
R R work R free
0.181 0.18 0.201
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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