› 6hym

X-ray diffraction
1.86Å resolution

Structure of PCM1 LIR motif bound to GABARAP

Released:
DOI: 10.2210/pdb6hym/pdb
PDB entry 6hym coloured by chain, front view.
PDB entry 6hym coloured by chain, side view.
PDB entry 6hym coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular determinants regulating selective binding of autophagy adapters and receptors to ATG8 proteins.
Wirth M, Zhang W, Razi M, Nyoni L, Joshi D, O'Reilly N, Johansen T, Tooze SA, Mouilleron S
Nat Commun 10 2055 (2019)
PMID: 31053714

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131837 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-aminobutyric acid receptor-associated protein; Pericentriolar material 1 protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 138 amino acids
Theoretical weight: 16.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15154 (Residues: 1959-1972; Coverage: 1%)
  • Canonical: O95166 (Residues: 1-117; Coverage: 100%)
Gene names: FLC3B, GABARAP, HT004, PCM1
Sequence domains: Autophagy protein Atg8 ubiquitin like

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P43
Unit cell:
a: 80.873Å b: 80.873Å c: 55.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.218
Expression system: Escherichia coli

Quick links

Citations

13 review citations

The Overcrowded Crossroads: Mitochondria, Alpha-Synuclein, and the Endo-Lysosomal System Interaction in Parkinson's Disease.
Lin et al. (2019)
12 more

2 mentions without citation

Molecular determinants regulating selective binding of autophagy adapters and receptors to ATG8 proteins.
Wirth et al. (2019)
1 more