› 6hzy

X-ray diffraction
1.7Å resolution

Crystal structure of a bacterial fucosidase with inhibitor FucPUG

Released:
DOI: 10.2210/pdb6hzy/pdb
PDB entry 6hzy coloured by chain, front view.
PDB entry 6hzy coloured by chain, side view.
PDB entry 6hzy coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Synthetic and Crystallographic Insight into Exploiting sp2 Hybridization in the Development of α-l-Fucosidase Inhibitors.
Coyle T, Wu L, Debowski AW, Davies GJ, Stubbs KA
Chembiochem 20 1365-1368 (2019)
PMID: 30663832

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183666 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-L-fucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 475 amino acids
Theoretical weight: 54.83 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8A3I4 (Residues: 31-484; Coverage: 100%)
Gene name: BT_2970
Sequence domains: Alpha-L-fucosidase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand EDO 17 x EDO
Ligand GYZ 2 x GYZ
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Unit cell:
a: 104.925Å b: 84.602Å c: 121.737Å
α: 90° β: 109° γ: 90°
R-values:
R R work R free
0.21 0.208 0.254
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The dual role of fucosidases: tool or target.
Jiménez-Pérez et al. (2023)
1 other article

2 mentions without citation

Origins and Evolution of the α-L-Fucosidases: From Bacteria to Metazoans.
You et al. (2019)
1 more