› 6i04

X-ray diffraction
3.1Å resolution

Crystal structure of Sema domain of the Met receptor in complex with FAB

Released:
DOI: 10.2210/pdb6i04/pdb
Model geometry
Fit model/data
PDB entry 6i04 coloured by chain, front view.
PDB entry 6i04 coloured by chain, side view.
PDB entry 6i04 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
MM-131, a bispecific anti-Met/EpCAM mAb, inhibits HGF-dependent and HGF-independent Met signaling through concurrent binding to EpCAM.
Casaletto JB, Geddie ML, Abu-Yousif AO, Masson K, Fulgham A, Boudot A, Maiwald T, Kearns JD, Kohli N, Su S, Razlog M, Raue A, Kalra A, Håkansson M, Logan DT, Welin M, Chattopadhyay S, Harms BD, Nielsen UB, Schoeberl B, Lugovskoy AA, MacBeath G
Proc Natl Acad Sci U S A 116 7533-7542 (2019)
PMID: 30898885

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279554 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Hepatocyte growth factor receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 552 amino acids
Theoretical weight: 62.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P08581 (Residues: 25-564; Coverage: 40%)
Gene name: MET
Sequence domains:
Fab heavy chain Chains: C, H
Molecule details ›
Chains: C, H
Length: 230 amino acids
Theoretical weight: 24.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fab light chain Chains: D, L

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P1
Unit cell:
a: 73.932Å b: 85.497Å c: 101.3Å
α: 77.49° β: 88.58° γ: 68.76°
R-values:
R R work R free
0.205 0.202 0.261
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Understanding the versatile roles and applications of EpCAM in cancers: from bench to bedside.
Liu et al. (2022)
6 more

2 mentions without citation

State of the structure address on MET receptor activation by HGF.
Linossi et al. (2021)
1 more