› 6i06

X-ray diffraction
2Å resolution

Crystal structure of psychrophilic phosphoglycerate kinase from Pseudomonas TACII18

Released:
DOI: 10.2210/pdb6i06/pdb
PDB entry 6i06 coloured by chain, front view.
PDB entry 6i06 coloured by chain, side view.
PDB entry 6i06 coloured by chain, top view.
Source organism: Pseudomonas
Primary publication:
Structural determinants increasing flexibility confer cold adaptation in psychrophilic phosphoglycerate kinase.
Mandelman D, Ballut L, Wolff DA, Feller G, Gerday C, Haser R, Aghajari N
Extremophiles 23 495-506 (2019)
PMID: 31147836

Function and Biology Details

Reaction catalysed: 
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193131 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoglycerate kinase Chain: A
Molecule details ›
Chain: A
Length: 387 amino acids
Theoretical weight: 40.3 KDa
Source organism: Pseudomonas
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RBS3 (Residues: 1-387; Coverage: 100%)
Gene name: pgk
Sequence domains: Phosphoglycerate kinase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P32
Unit cell:
a: 59.1Å b: 59.1Å c: 87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.179 0.229
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Industrial applications of cold-adapted enzymes: challenges, innovations and future perspective.
Kumar et al. (2021)
1 more

1 mention without citation

Phosphoglycerate kinase: structural aspects and functions, with special emphasis on the enzyme from Kinetoplastea.
Rojas-Pirela et al. (2020)