› 6i22

X-ray diffraction
1.66Å resolution

Flavin Analogue Sheds Light on Light-Oxygen-Voltage Domain Mechanism

Released:
DOI: 10.2210/pdb6i22/pdb
PDB entry 6i22 coloured by chain, front view.
PDB entry 6i22 coloured by chain, side view.
PDB entry 6i22 coloured by chain, top view.
Source organism: Ochromonas danica
Primary publication:
A Noncanonical Chromophore Reveals Structural Rearrangements of the Light-Oxygen-Voltage Domain upon Photoactivation.
Kalvaitis ME, Johnson LA, Mart RJ, Rizkallah P, Allemann RK
Biochemistry 58 2608-2616 (2019)
PMID: 31082213

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aureochrome1-like protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 134 amino acids
Theoretical weight: 14.88 KDa
Source organism: Ochromonas danica
Expression system: Escherichia coli
UniProt:
  • Canonical: C5NSW6 (Residues: 181-311; Coverage: 42%)
Gene name: OdAUREO1
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand FMN 4 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 150.18Å b: 91.51Å c: 99.84Å
α: 90° β: 131.34° γ: 90°
R-values:
R R work R free
0.204 0.203 0.221
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Signal transduction in photoreceptor histidine kinases.
Möglich A. (2019)
6 other articles

1 mention without citation

A Noncanonical Chromophore Reveals Structural Rearrangements of the Light-Oxygen-Voltage Domain upon Photoactivation.
Kalvaitis et al. (2019)