› 6i7g

X-ray diffraction
2.7Å resolution

Crystal structure of dimeric wild type FICD complexed with ATP

Released:
DOI: 10.2210/pdb6i7g/pdb
PDB entry 6i7g coloured by chain, front view.
PDB entry 6i7g coloured by chain, side view.
PDB entry 6i7g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An oligomeric state-dependent switch in the ER enzyme FICD regulates AMPylation and deAMPylation of BiP.
Perera LA, Rato C, Yan Y, Neidhardt L, McLaughlin SH, Read RJ, Preissler S, Ron D
EMBO J 38 e102177 (2019)
PMID: 31531998

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein adenylyltransferase FICD Chains: A, B
Molecule details ›
Chains: A, B
Length: 343 amino acids
Theoretical weight: 39.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9BVA6 (Residues: 104-445; Coverage: 75%)
Gene names: FICD, HIP13, HYPE, UNQ3041/PRO9857
Sequence domains: Fic/DOC family

Ligands and Environments

4 bound ligands:
Ligand ATP 2 x ATP
Ligand PEG 1 x PEG
Ligand 1PE 2 x 1PE
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21212
Unit cell:
a: 77.671Å b: 107.652Å c: 132.597Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.285 0.283 0.319
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Post-translational modifications of Hsp70 family proteins: Expanding the chaperone code.
Nitika et al. (2020)
3 more

4 mentions without citation

An oligomeric state-dependent switch in the ER enzyme FICD regulates AMPylation and deAMPylation of BiP.
Perera et al. (2019)
3 more