› 6i8g

X-ray diffraction
2.34Å resolution

Structure of the plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody ENB73

Released:
DOI: 10.2210/pdb6i8g/pdb
PDB entry 6i8g coloured by chain, front view.
PDB entry 6i8g coloured by chain, side view.
PDB entry 6i8g coloured by chain, top view.
Source organisms:
Primary publication:
Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.
Voss M, Toelzer C, Bhandari DD, Parker JE, Niefind K
J Struct Biol 208 107390 (2019)
PMID: 31550533

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212865 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein EDS1L Chain: A
Molecule details ›
Chain: A
Length: 631 amino acids
Theoretical weight: 72.74 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9XF23 (Residues: 1-623; Coverage: 100%)
Gene name: EDS1
Sequence domains:
EDS1-specific nanobody Chain: B
Molecule details ›
Chain: B
Length: 143 amino acids
Theoretical weight: 15.88 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: C2
Unit cell:
a: 177.504Å b: 68.232Å c: 105.273Å
α: 90° β: 123.44° γ: 90°
R-values:
R R work R free
0.195 0.194 0.222
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Molecular innovations in plant TIR-based immunity signaling.
Lapin et al. (2022)
3 more

1 mention without citation

Applications of nanobodies in plant science and biotechnology.
Wang et al. (2021)