› 6i9h

Solution NMR

Solution structure of TRIM28 RING domain

Released:
DOI: 10.2210/pdb6i9h/pdb
PDB entry 6i9h coloured by chain, ensemble of 18 models, front view.
PDB entry 6i9h coloured by chain, ensemble of 18 models, side view.
PDB entry 6i9h coloured by chain, ensemble of 18 models, top view.
Source organism: Homo sapiens
Primary publication:
Characterisation of class VI TRIM RING domains: linking RING activity to C-terminal domain identity.
Stevens RV, Esposito D, Rittinger K
Life Sci Alliance 2 (2019)
PMID: 31028095

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription intermediary factor 1-beta Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 9.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13263 (Residues: 54-145; Coverage: 11%)
Gene names: KAP1, RNF96, TIF1B, TRIM28
Sequence domains: zinc-RING finger domain

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 91%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

5 review citations

TIF1 Proteins in Genome Stability and Cancer.
McAvera et al. (2020)
4 more

6 mentions without citation

Targeting TRIM Proteins: A Quest towards Drugging an Emerging Protein Class.
D'Amico et al. (2021)
5 more