› 6iam

X-ray diffraction
1.51Å resolution

Modulating Protein-Protein Interactions with Visible Light Peptide Backbone Switches

Released:
DOI: 10.2210/pdb6iam/pdb
PDB entry 6iam coloured by chain, front view.
PDB entry 6iam coloured by chain, side view.
PDB entry 6iam coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Modulating Protein-Protein Interactions with Visible-Light-Responsive Peptide Backbone Photoswitches.
Albert L, Peñalver A, Djokovic N, Werel L, Hoffarth M, Ruzic D, Xu J, Essen LO, Nikolic K, Dou Y, Vázquez O
Chembiochem 20 1417-1429 (2019)
PMID: 30675988

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-124313 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
WD repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 33.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61964 (Residues: 29-334; Coverage: 92%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
SER-ALA-ARG-ALA-XY5-VAL-HIS-LEU-ARG-LYS-SER-ALA Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.46 KDa
Source organism: Homo sapiens
Expression system: Not provided
Small ubiquitin-related modifier 5 Chain: C
Molecule details ›
Chain: C
Length: 4 amino acids
Theoretical weight: 445 Da
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G2XKQ0 (Residues: 5-8; Coverage: 4%)
Gene names: SUMO1P1, SUMO5, UBL2, UBL6

Ligands and Environments

2 bound ligands:
Ligand K 3 x K
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 46.526Å b: 46.56Å c: 66.168Å
α: 90° β: 107.22° γ: 90°
R-values:
R R work R free
0.148 0.148 0.17
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Protein-protein interaction modulators: advances, successes and remaining challenges.
Mabonga et al. (2019)
4 more