› 6iav

X-ray diffraction
2Å resolution

CO-AZURIN FROM PSEUDOMONAS AERUGINOSA TREATED WITH HYDROSULFIDE

Released:
DOI: 10.2210/pdb6iav/pdb
PDB entry 6iav coloured by chain, front view.
PDB entry 6iav coloured by chain, side view.
PDB entry 6iav coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Azurin and HS-: towards implementation of a sensor for HS- detection.
Strianese M, Palm GJ, Kohlhause D, Ndamba LA, Tabares LC, Pellecchia C
Eur. J. inorg. Chem. (2019)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 127 amino acids
Theoretical weight: 13.89 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00282 (Residues: 22-148; Coverage: 99%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family

Ligands and Environments

2 bound ligands:
Ligand CO 4 x CO
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P21
Unit cell:
a: 50.429Å b: 59.95Å c: 82.52Å
α: 90° β: 90.43° γ: 90°
R-values:
R R work R free
0.253 0.25 0.31
Expression system: Escherichia coli BL21

Quick links