› 6ici

X-ray diffraction
2.3Å resolution

Crystal structure of human MICAL3

Released:
DOI: 10.2210/pdb6ici/pdb
PDB entry 6ici coloured by chain, front view.
PDB entry 6ici coloured by chain, side view.
PDB entry 6ici coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and kinetic insights into flavin-containing monooxygenase and calponin-homology domains in human MICAL3.
Kim J, Lee H, Roh YJ, Kim HU, Shin D, Kim S, Son J, Lee A, Kim M, Park J, Hwang SY, Kim K, Lee YK, Jung HS, Hwang KY, Lee BC
IUCrJ 7 90-99 (2020)
PMID: 31949908

Function and Biology Details

Reaction catalysed: 
[F-actin]-L-methionine + NADPH + O(2) + H(+) = [F-actin]-L-methionine-(R)-S-oxide + NADP(+) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181906 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
[F-actin]-monooxygenase MICAL3 Chain: A
Molecule details ›
Chain: A
Length: 736 amino acids
Theoretical weight: 83.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7RTP6 (Residues: 1-700; Coverage: 35%)
Gene names: KIAA0819, KIAA1364, MICAL3
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 65.211Å b: 93.466Å c: 71.568Å
α: 90° β: 92.366° γ: 90°
R-values:
R R work R free
0.174 0.172 0.214
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structural Characteristics, Binding Partners and Related Diseases of the Calponin Homology (CH) Domain.
Yin et al. (2020)
2 more

1 mention without citation

Structural and kinetic insights into flavin-containing monooxygenase and calponin-homology domains in human MICAL3.
Kim et al. (2020)