› 6id4

X-ray diffraction
2.4Å resolution

Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01

Released:
DOI: 10.2210/pdb6id4/pdb
PDB entry 6id4 coloured by chain, front view.
PDB entry 6id4 coloured by chain, side view.
PDB entry 6id4 coloured by chain, top view.
Source organisms:
Primary publication:
Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01.
Gu Y, Wong YH, Liew CW, Chan CEZ, Murali TM, Yap J, Too CT, Purushotorman K, Hamidinia M, El Sahili A, Goh ATH, Teo RZC, Wood KJ, Hanson BJ, Gascoigne NRJ, Lescar J, Vathsala A, MacAry PA
Nat Commun 10 893 (2019)
PMID: 30792391

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-207856 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Ig-like domain-containing protein Chains: A, E
Molecule details ›
Chains: A, E
Length: 276 amino acids
Theoretical weight: 31.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: F6IQY1 (Residues: 25-299; Coverage: 88%)
Gene name: HLA-A
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, F
Molecule details ›
Chains: B, F
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Heavy chain Chains: C, H
Molecule details ›
Chains: C, H
Length: 222 amino acids
Theoretical weight: 23.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Light chain Chains: D, L
Molecule details ›
Chains: D, L
Length: 221 amino acids
Theoretical weight: 23.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
peptide Chains: T, U
Molecule details ›
Chains: T, U
Length: 9 amino acids
Theoretical weight: 1.01 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand GOL 5 x GOL
Ligand PEG 5 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: C2
Unit cell:
a: 124.56Å b: 215.33Å c: 80.98Å
α: 90° β: 92.96° γ: 90°
R-values:
R R work R free
0.199 0.197 0.246
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 citation in other articles

A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Kongsuphol et al. (2021)
7 more

9 mentions without citation

Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01.
Gu et al. (2019)
8 more