› 6ieg

X-ray diffraction
3.55Å resolution

Crystal structure of human MTR4

Released:
DOI: 10.2210/pdb6ieg/pdb
PDB entry 6ieg coloured by chain, front view.
PDB entry 6ieg coloured by chain, side view.
PDB entry 6ieg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction.
Wang J, Chen J, Wu G, Zhang H, Du X, Chen S, Zhang L, Wang K, Fan J, Gao S, Wu X, Zhang S, Kuai B, Zhao P, Chi B, Wang L, Li G, Wong CCL, Zhou Y, Li J, Yun C, Cheng H
Genes Dev 33 536-549 (2019)
PMID: 30842217

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154594 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exosome RNA helicase MTR4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1002 amino acids
Theoretical weight: 114.16 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P42285 (Residues: 71-1042; Coverage: 93%)
Gene names: DOB1, KIAA0052, MTR4, MTREX, SKIV2L2
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 86.316Å b: 119.139Å c: 124.567Å
α: 90° β: 99.87° γ: 90°
R-values:
R R work R free
0.27 0.268 0.305
Expression system: Spodoptera frugiperda

Quick links

Citations

5 review citations

Regulation of exosome production and cargo sorting.
Wei et al. (2021)
4 more

3 mentions without citation

Mtr4 RNA helicase structures and interactions.
Olsen et al. (2021)
2 more