› 6ieu

X-ray diffraction
1.79Å resolution

The structure of TRIM66 PHD-Bromo domain with unmodified H3 N terminal peptide

Released:
DOI: 10.2210/pdb6ieu/pdb
PDB entry 6ieu coloured by chain, front view.
PDB entry 6ieu coloured by chain, side view.
PDB entry 6ieu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
TRIM66 reads unmodified H3R2K4 and H3K56ac to respond to DNA damage in embryonic stem cells.
Chen J, Wang Z, Guo X, Li F, Wei Q, Chen X, Gong D, Xu Y, Chen W, Liu Y, Kang J, Shi Y
Nat Commun 10 4273 (2019)
PMID: 31537782

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127263 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tripartite motif-containing protein 66 Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 22.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15016 (Residues: 1103-1295; Coverage: 14%)
Gene names: C11orf29, KIAA0298, TRIM66
Sequence domains:
Histone H3.1 Chain: C
Molecule details ›
Chain: C
Length: 12 amino acids
Theoretical weight: 1.31 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-13; Coverage: 9%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 100.266Å b: 63.465Å c: 33.303Å
α: 90° β: 101.67° γ: 90°
R-values:
R R work R free
0.188 0.186 0.216
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

TIF1 Proteins in Genome Stability and Cancer.
McAvera et al. (2020)
7 more