6if2

X-ray diffraction
2.4Å resolution

Complex structure of Rab35 and its effector RUSC2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-172247 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rab-35 Chain: B
Molecule details ›
Chain: B
Length: 186 amino acids
Theoretical weight: 21.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15286 (Residues: 1-180; Coverage: 90%)
Gene names: RAB1C, RAB35, RAY
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
AP-4 complex accessory subunit RUSC2 Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 22.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8N2Y8 (Residues: 983-1181; Coverage: 13%)
Gene names: KIAA0375, RUSC2
Sequence domains: RUN domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P65
Unit cell:
a: 188.213Å b: 188.213Å c: 37.185Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.167 0.204
Expression system: Escherichia coli BL21(DE3)