› 6ig2

X-ray diffraction
2.88Å resolution

Structure of mitochondrial CDP-DAG synthase Tam41 complexed with CTP, delta 74, F240A

Released:
DOI: 10.2210/pdb6ig2/pdb
PDB entry 6ig2 coloured by chain, front view.
PDB entry 6ig2 coloured by chain, side view.
PDB entry 6ig2 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Structures of the Mitochondrial CDP-DAG Synthase Tam41 Suggest a Potential Lipid Substrate Pathway from Membrane to the Active Site.
Jiao H, Yin Y, Liu Z
Structure 27 1258-1269.e4 (2019)
PMID: 31178220

Function and Biology Details

Reaction catalysed: 
CTP + phosphatidate = diphosphate + CDP-diacylglycerol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130779 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidate cytidylyltransferase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 313 amino acids
Theoretical weight: 35.86 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O74339 (Residues: 28-319; Coverage: 74%)
Gene names: SPBC1A4.06c, tam41
Sequence domains: Phosphatidate cytidylyltransferase, mitochondrial

Ligands and Environments

1 bound ligand:
Ligand CTP 1 x CTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Unit cell:
a: 55.266Å b: 187.418Å c: 186.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.259
Expression system: Escherichia coli

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Citations

2 review citations

Protein Amphipathic Helix Insertion: A Mechanism to Induce Membrane Fission.
Zhukovsky et al. (2019)
1 more