› 6ihw

X-ray diffraction
1.55Å resolution

Crystal structure of bacterial serine phosphatase bearing R161K mutation

Released:
DOI: 10.2210/pdb6ihw/pdb
PDB entry 6ihw coloured by chain, front view.
PDB entry 6ihw coloured by chain, side view.
PDB entry 6ihw coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural Insight into the Mechanism of Staphylococcus aureus Stp1 Phosphatase.
Yang T, Liu T, Gan J, Yu K, Chen K, Xue W, Lan L, Yang S, Yang CG
ACS Infect Dis 5 841-850 (2019)
PMID: 30868877

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193196 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
protein-serine/threonine phosphatase Chain: A
Molecule details ›
Chain: A
Length: 266 amino acids
Theoretical weight: 30.11 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RL81 (Residues: 3-247; Coverage: 99%)
Gene names: A6760_05000, BN1321_240063, EP54_08495, EQ90_08165, FAF17_11265, GO793_10355, GO814_06360, GO942_07250, GZ163_05055, NCTC10702_01924, NCTC13131_00423, NCTC7972_00731, SAMEA2078260_01528, SAMEA2078588_01607, SAMEA2080344_01697, SAMEA2081063_01376, SAMEA4008575_01865, SAMEA70146418_01691, SAMEA70245418_01977, prpC, prpC_1
Sequence domains: Protein phosphatase 2C

Ligands and Environments

1 bound ligand:
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 46.578Å b: 38.958Å c: 65.409Å
α: 90° β: 102.08° γ: 90°
R-values:
R R work R free
0.152 0.151 0.174
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Insight into the Dual Inhibition Mechanism of Corilagin against MRSA Serine/Threonine Phosphatase (Stp1) by Molecular Modeling.
Yang et al. (2020)
1 more