› 6ii2

X-ray diffraction
3.5Å resolution

Crystal structure of alpha-beta hydrolase (ABH) and Makes Caterpillars Floppy (MCF)-Like effectors of Vibrio vulnificus MO6-24/O

Released:
DOI: 10.2210/pdb6ii2/pdb
PDB entry 6ii2 coloured by chain, front view.
PDB entry 6ii2 coloured by chain, side view.
PDB entry 6ii2 coloured by chain, top view.
Source organism: Vibrio vulnificus
Primary publication:
Makes caterpillars floppy-like effector-containing MARTX toxins require host ADP-ribosylation factor (ARF) proteins for systemic pathogenicity.
Lee Y, Kim BS, Choi S, Lee EY, Park S, Hwang J, Kwon Y, Hyun J, Lee C, Kim JF, Eom SH, Kim MH
Proc Natl Acad Sci U S A 116 18031-18040 (2019)
PMID: 31427506

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase C80 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 694 amino acids
Theoretical weight: 75.16 KDa
Source organism: Vibrio vulnificus
Expression system: Escherichia coli
UniProt:
  • Canonical: F1CLG9 (Residues: 995-1681; Coverage: 37%)
Gene name: rtxA1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P212121
Unit cell:
a: 86.215Å b: 108.593Å c: 334.737Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.287 0.285 0.327
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Spatiotemporal Regulation of Vibrio Exotoxins by HlyU and Other Transcriptional Regulators.
Kim BS. (2020)
2 more

1 mention without citation

Makes caterpillars floppy-like effector-containing MARTX toxins require host ADP-ribosylation factor (ARF) proteins for systemic pathogenicity.
Lee et al. (2019)