› 6iih

X-ray diffraction
1.96Å resolution

crystal structure of mitochondrial calcium uptake 2(MICU2)

Released:
DOI: 10.2210/pdb6iih/pdb
PDB entry 6iih coloured by chain, front view.
PDB entry 6iih coloured by chain, side view.
PDB entry 6iih coloured by chain, top view.
Source organisms:
Primary publication:
The crystal structure of MICU2 provides insight into Ca2+ binding and MICU1-MICU2 heterodimer formation.
Wu W, Shen Q, Lei Z, Qiu Z, Li D, Pei H, Zheng J, Jia Z
EMBO Rep 20 e47488 (2019)
PMID: 31397067

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium uptake protein 2, mitochondrial; Endolysin Chains: A, B
Molecule details ›
Chains: A, B
Length: 493 amino acids
Theoretical weight: 57.74 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00720 (Residues: 1-161; Coverage: 98%)
  • Canonical: Q8IYU8 (Residues: 85-406; Coverage: 74%)
Gene names: E, EFHA1, MICU2
Sequence domains: Phage lysozyme

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 66.743Å b: 96.311Å c: 85.488Å
α: 90° β: 98.98° γ: 90°
R-values:
R R work R free
0.181 0.18 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Mitochondrial calcium exchange in physiology and disease.
Garbincius et al. (2022)
7 more

5 mentions without citation

Structure and mechanism of the mitochondrial Ca2+ uniporter holocomplex.
Fan et al. (2020)
4 more