› 6iis

X-ray diffraction
2.36Å resolution

Complex structure of the HRP3 PWWP domain with both a 16-bp TA-rich DNA and an H3K36me3-containing histone peptide

Released:
DOI: 10.2210/pdb6iis/pdb
PDB entry 6iis coloured by chain, front view.
PDB entry 6iis coloured by chain, side view.
PDB entry 6iis coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Complex structure of the HRP3 PWWP domain with both a 16-bp TA-rich DNA and an H3K36me3-containing histone peptide
Wang Z, Tian W
Nucleic Acids Res. (2019)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120014 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
Hepatoma-derived growth factor-related protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 100 amino acids
Theoretical weight: 11.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y3E1 (Residues: 1-99; Coverage: 49%)
Gene names: CGI-142, HDGF2, HDGFL3, HDGFRP3
Sequence domains: PWWP domain
Histone H3.1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 8 amino acids
Theoretical weight: 924 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 34-41; Coverage: 6%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
16-bp TA-rich DNA Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 nucleotides
Theoretical weight: 4.89 KDa

Ligands and Environments

No bound ligands
1 modified residue:
Ligand M3L 2 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P31
Unit cell:
a: 33.473Å b: 33.473Å c: 198.774Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.186 0.263
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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