› 6ij6

X-ray diffraction
1.95Å resolution

Crystal structure of PETase S121E, D186H, R280A mutant from Ideonella sakaiensis

Released:
DOI: 10.2210/pdb6ij6/pdb
PDB entry 6ij6 coloured by chain, front view.
PDB entry 6ij6 coloured by chain, side view.
PDB entry 6ij6 coloured by chain, top view.
Source organism: Ideonella sakaiensis
Primary publication:
Rational Protein Engineering of Thermo-Stable PETase from Ideonella sakaiensis for Highly Efficient PET Degradation
Son HF, Cho IJ, Joo S, Seo H, Sagong HY, Choi SY, Lee SY, Kim KJ
ACS Catalysis 9 3519-3526 (2019)

Function and Biology Details

Reaction catalysed: 
(Ethylene terephthalate)(n) + H(2)O = (ethylene terephthalate)(n-1) + ethylene terephthalate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102331 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(ethylene terephthalate) hydrolase Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 31.5 KDa
Source organism: Ideonella sakaiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0K8P6T7 (Residues: 34-290; Coverage: 98%)
Gene name: ISF6_4831
Sequence domains: Dienelactone hydrolase family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: C2
Unit cell:
a: 114.913Å b: 51.14Å c: 51.157Å
α: 90° β: 109.68° γ: 90°
R-values:
R R work R free
0.153 0.151 0.193
Expression system: Escherichia coli

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