› 6im7

X-ray diffraction
1.97Å resolution

CueO-12.1 multicopper oxidase

Released:
DOI: 10.2210/pdb6im7/pdb
Model geometry
Fit model/data
PDB entry 6im7 coloured by chain, front view.
PDB entry 6im7 coloured by chain, side view.
PDB entry 6im7 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Development and structural characterization of an engineered multi-copper oxidase reporter of protein-protein interactions.
Sana B, Chee SMQ, Wongsantichon J, Raghavan S, Robinson RC, Ghadessy FJ
OpenAccess logo J Biol Chem 294 7002-7012 (2019)
PMID: 30770473

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multicopper oxidase CueO Chain: A
Molecule details ›
Chain: A
Length: 510 amino acids
Theoretical weight: 56.13 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36649 (Residues: 29-516; Coverage: 100%)
Gene names: JW0119, b0123, cueO, yacK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2
Unit cell:
a: 76.111Å b: 100.731Å c: 59.044Å
α: 90° β: 96.165° γ: 90°
R-values:
R R work R free
0.142 0.14 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Directed co-evolution of interacting protein-peptide pairs by compartmentalized two-hybrid replication (C2HR).
Siau et al. (2020)
1 more

1 mention without citation

Development and structural characterization of an engineered multi-copper oxidase reporter of protein-protein interactions.
Sana et al. (2019)