› 6ipb

X-ray diffraction
1.78Å resolution

Crystal Structure of the Cofactor-binding Domain of the Human Phase II Drug Metabolism Enzyme UGT2B15

Released:
DOI: 10.2210/pdb6ipb/pdb
PDB entry 6ipb coloured by chain, front view.
PDB entry 6ipb coloured by chain, side view.
PDB entry 6ipb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insight into tartrate inhibition patterns in vitro and in vivo based on cocrystal structure with UDP-glucuronosyltransferase 2B15.
Zhang L, Zhu L, Qu W, Wu F, Hu M, Xie W, Liu Z, Wang C
Biochem Pharmacol 172 113753 (2020)
PMID: 31837310

Function and Biology Details

Reaction catalysed: 
UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157115 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucuronosyltransferase 2B15 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 191 amino acids
Theoretical weight: 21.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54855 (Residues: 284-451; Coverage: 33%)
Gene names: UGT2B15, UGT2B8
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase

Ligands and Environments

2 bound ligands:
Ligand TLA 4 x TLA
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 50.067Å b: 141.824Å c: 56.884Å
α: 90° β: 115.58° γ: 90°
R-values:
R R work R free
0.147 0.146 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Application of the Nicoya OpenSPR to Studies of Biomolecular Binding: A Review of the Literature from 2016 to 2022.
Hanson et al. (2023)
3 other articles

2 mentions without citation

Complete Reaction Phenotyping of Propranolol and 4-Hydroxypropranolol with the 19 Enzymes of the Human UGT1 and UGT2 Families.
Yang et al. (2022)
1 more