6iro

Electron Microscopy
3.4Å resolution

the crosslinked complex of ISWI-nucleosome in the ADP-bound state

Released:
Primary publication:
Structures of the ISWI-nucleosome complex reveal a conserved mechanism of chromatin remodeling.
Nat Struct Mol Biol 26 258-266 (2019)
PMID: 30872815
Related structures: EMD-9720

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-120031 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
ISWI chromatin-remodeling complex ATPase ISW1 Chain: L
Molecule details ›
Chain: L
Length: 1061 amino acids
Theoretical weight: 123.18 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P38144 (Residues: 69-1129; Coverage: 94%)
Gene names: ISW1, YBR1633, YBR245C
Sequence domains:
Histone H3.2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 135 amino acids
Theoretical weight: 15.3 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P84233 (Residues: 2-136; Coverage: 99%)
Sequence domains: Core histone H2A/H2B/H3/H4
Structure domains: Histone, subunit A
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 102 amino acids
Theoretical weight: 11.26 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P62799 (Residues: 2-103; Coverage: 99%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Structure domains: Histone, subunit A
Histone H2A type 1 Chains: C, G
Molecule details ›
Chains: C, G
Length: 129 amino acids
Theoretical weight: 13.98 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P06897 (Residues: 2-130; Coverage: 99%)
Sequence domains:
Structure domains: Histone, subunit A
Histone H2B 1.1 Chains: D, H
Molecule details ›
Chains: D, H
Length: 122 amino acids
Theoretical weight: 13.52 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P02281 (Residues: 5-126; Coverage: 97%)
Sequence domains: Core histone H2A/H2B/H3/H4
Structure domains: Histone, subunit A
DNA (167-MER) Chain: I
Molecule details ›
Chain: I
Length: 167 nucleotides
Theoretical weight: 51.36 KDa
DNA (167-MER) Chain: J
Molecule details ›
Chain: J
Length: 167 nucleotides
Theoretical weight: 51.75 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-9720
Expression system: Escherichia coli K-12