› 6ist

X-ray diffraction
1.75Å resolution

Crystal structure of a wild type endolysin LysIME-EF1

Released:
DOI: 10.2210/pdb6ist/pdb
PDB entry 6ist coloured by chain, front view.
PDB entry 6ist coloured by chain, side view.
PDB entry 6ist coloured by chain, top view.
Source organism: Enterococcus phage IMEEF1
Primary publication:
Structural and functional insights into a novel two-component endolysin encoded by a single gene in Enterococcus faecalis phage.
Zhou B, Zhen X, Zhou H, Zhao F, Fan C, Perčulija V, Tong Y, Mi Z, Ouyang S
PLoS Pathog 16 e1008394 (2020)
PMID: 32176738

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197438 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peptidase C51 domain-containing protein Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 70 amino acids
Theoretical weight: 8.17 KDa
Source organism: Enterococcus phage IMEEF1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: S5MRN1 (Residues: 168-237; Coverage: 30%)
Peptidase C51 domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 237 amino acids
Theoretical weight: 26.28 KDa
Source organism: Enterococcus phage IMEEF1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: S5MRN1 (Residues: 1-237; Coverage: 100%)
Sequence domains: CHAP domain

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 47.03Å b: 56.92Å c: 91.52Å
α: 90° β: 96.29° γ: 90°
R-values:
R R work R free
0.182 0.18 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Phage-Encoded Endolysins.
Abdelrahman et al. (2021)
3 more

3 mentions without citation

Structural and functional insights into a novel two-component endolysin encoded by a single gene in Enterococcus faecalis phage.
Zhou et al. (2020)
2 more