6itc

Electron Microscopy
3.45Å resolution

Structure of a substrate engaged SecA-SecY protein translocation machine

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-207586 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Protein translocase subunit SecA Chain: A
Molecule details ›
Chain: A
Length: 780 amino acids
Theoretical weight: 88.92 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli #1/H766
UniProt:
  • Canonical: P28366 (Residues: 1-780; Coverage: 93%)
Gene names: BSU35300, div+, secA
Sequence domains:
Protein translocase subunit SecY Chain: Y
Molecule details ›
Chain: Y
Length: 424 amino acids
Theoretical weight: 46.77 KDa
Source organism: Geobacillus thermodenitrificans NG80-2
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A4IJK8 (Residues: 1-430; Coverage: 99%)
Gene names: GTNG_0125, secY
Sequence domains: SecY
Protein translocase subunit SecE Chain: E
Molecule details ›
Chain: E
Length: 70 amino acids
Theoretical weight: 8.25 KDa
Source organism: Geobacillus thermodenitrificans NG80-2
Expression system: Escherichia coli
UniProt:
  • Canonical: A4IJH4 (Residues: 1-60; Coverage: 100%)
Gene names: GTNG_0091, secE
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex
Nanobody Chain: V
Molecule details ›
Chain: V
Length: 116 amino acids
Theoretical weight: 12.92 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Outer membrane protein A Chain: B
Molecule details ›
Chain: B
Length: 59 amino acids
Theoretical weight: 6.02 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A910 (Residues: 2-26; Coverage: 2%)
Gene names: JW0940, b0957, con, ompA, tolG, tut
Green fluorescent protein Chain: G
Molecule details ›
Chain: G
Length: 236 amino acids
Theoretical weight: 26.81 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Nanobody Chain: C
Molecule details ›
Chain: C
Length: 112 amino acids
Theoretical weight: 12.37 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.45Å
Relevant EMDB volumes: EMD-9731
Expression systems:
  • Escherichia coli #1/H766
  • Escherichia coli K-12
  • Escherichia coli