› 6itu

X-ray diffraction
2.17Å resolution

Crystal Structure of the GULP1 PTB domain-APP peptide complex

Released:
DOI: 10.2210/pdb6itu/pdb
PDB entry 6itu coloured by chain, front view.
PDB entry 6itu coloured by chain, side view.
PDB entry 6itu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Attenuation of amyloid-β generation by atypical protein kinase C-mediated phosphorylation of engulfment adaptor PTB domain containing 1 threonine 35.
Chau DD, Yung KW, Chan WW, An Y, Hao Y, Chan HE, Ngo JC, Lau KF
FASEB J 33 12019-12035 (2019)
PMID: 31373844

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138372 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PTB domain-containing engulfment adapter protein 1 Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 19.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UBP9 (Residues: 1-168; Coverage: 55%)
Gene names: CED6, GULP, GULP1
Sequence domains: Phosphotyrosine interaction domain (PTB/PID)
C31 Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.51 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P05067 (Residues: 755-766; Coverage: 2%)
Gene names: A4, AD1, APP

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P3221
Unit cell:
a: 63.91Å b: 63.91Å c: 108.771Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.197 0.221
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 citation in other articles

ARNO is recruited by the neuronal adaptor FE65 to potentiate ARF6-mediated neurite outgrowth.
Zhai et al. (2022)
1 more

3 mentions without citation

Attenuation of amyloid-β generation by atypical protein kinase C-mediated phosphorylation of engulfment adaptor PTB domain containing 1 threonine 35.
Chau et al. (2019)
2 more