› 6iu7

X-ray diffraction
1.9Å resolution

Crystal structure of importin-alpha1 bound to the 53BP1 nuclear localization signal (wild-type)

Released:
DOI: 10.2210/pdb6iu7/pdb
Model geometry
Fit model/data
PDB entry 6iu7 coloured by chain, front view.
PDB entry 6iu7 coloured by chain, side view.
PDB entry 6iu7 coloured by chain, top view.
Source organisms:
Primary publication:
Structural and biochemical characterization of the recognition of the 53BP1 nuclear localization signal by importin-α.
Matsuura Y
Biochem Biophys Res Commun 510 236-241 (2019)
PMID: 30685087

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156519 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 427 amino acids
Theoretical weight: 46.24 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 72-498; Coverage: 81%)
Gene names: Kpna2, Rch1
Sequence domains:
TP53-binding protein 1 Chain: B
Molecule details ›
Chain: B
Length: 22 amino acids
Theoretical weight: 2.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12888 (Residues: 1665-1686; Coverage: 1%)
Gene name: TP53BP1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 79.46Å b: 90.15Å c: 96.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.185
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Types of nuclear localization signals and mechanisms of protein import into the nucleus.
Lu et al. (2021)
3 more

2 mentions without citation

Comparative study of the interactions between fungal transcription factor nuclear localization sequences with mammalian and fungal importin-alpha.
Bernardes et al. (2020)
1 more