› 6iv6

Electron Microscopy
3.6Å resolution

Cryo-EM structure of AcrVA5-acetylated MbCas12a in complex with crRNA

Released:
DOI: 10.2210/pdb6iv6/pdb
PDB entry 6iv6 coloured by chain, front view.
PDB entry 6iv6 coloured by chain, side view.
PDB entry 6iv6 coloured by chain, top view.
Source organisms:
Primary publication:
An anti-CRISPR protein disables type V Cas12a by acetylation.
Dong L, Guan X, Li N, Zhang F, Zhu Y, Ren K, Yu L, Zhou F, Han Z, Gao N, Huang Z
Nat Struct Mol Biol 26 308-314 (2019)
PMID: 30936526
Related structures: EMD-9742

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102733 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Type V CRISPR-associated protein Cpf1 Chain: A
Molecule details ›
Chain: A
Length: 1261 amino acids
Theoretical weight: 145.25 KDa
Source organism: Moraxella bovoculi
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0U2B2X7 (Residues: 1-1261; Coverage: 100%)
Gene name: AAX07_00205
Sequence domains:
RNA (59-MER) Chain: G
Molecule details ›
Chain: G
Length: 59 nucleotides
Theoretical weight: 18.67 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand ALY 4 x ALY

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-9742
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

21 review citations

The arms race between bacteria and their phage foes.
Hampton et al. (2020)
20 more

1 mention without citation

Structures and Strategies of Anti-CRISPR-Mediated Immune Suppression.
Wiegand et al. (2020)