› 6j2s

X-ray diffraction
1.73Å resolution

Structure of HitA bound to gallium from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb6j2s/pdb
PDB entry 6j2s coloured by chain, front view.
PDB entry 6j2s coloured by chain, side view.
PDB entry 6j2s coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Identification and Characterization of a Metalloprotein Involved in Gallium Internalization in Pseudomonas aeruginosa.
Guo Y, Li W, Li H, Xia W
ACS Infect Dis 5 1693-1697 (2019)
PMID: 31475514

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191000 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferric iron-binding periplasmic protein HitA Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 33.21 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HVA8 (Residues: 29-335; Coverage: 100%)
Gene names: PA4687, hitA
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand GA 2 x GA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 50.93Å b: 100.46Å c: 58.54Å
α: 90° β: 109.55° γ: 90°
R-values:
R R work R free
0.178 0.176 0.209
Expression system: Escherichia coli

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Citations

1 review citation

Metallotherapeutics development in the age of iron-clad bacteria.
Centola et al. (2020)
3 other articles