› 6j4r

X-ray diffraction
2.8Å resolution

Structural basis for the target DNA recognition and binding by the MYB domain of phosphate starvation response regulator 1

Released:
DOI: 10.2210/pdb6j4r/pdb
PDB entry 6j4r coloured by chain, front view.
PDB entry 6j4r coloured by chain, side view.
PDB entry 6j4r coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the Target DNA recognition and binding by the MYB domain of phosphate starvation response 1.
Jiang M, Sun L, Isupov MN, Littlechild JA, Wu X, Wang Q, Wang Q, Yang W, Wu Y
FEBS J 286 2809-2821 (2019)
PMID: 30974511

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120054 (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
Protein PHOSPHATE STARVATION RESPONSE 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 58 amino acids
Theoretical weight: 6.7 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q94CL7 (Residues: 225-282; Coverage: 14%)
Gene names: At4g28610, PHR1, T5F17.60
Sequence domains: Myb-like DNA-binding domain
Structure domains: Homeodomain-like
DNA (5'-D(*CP*CP*AP*TP*AP*TP*AP*TP*GP*AP*C)-3') Chain: F
Molecule details ›
Chain: F
Length: 11 nucleotides
Theoretical weight: 3.32 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*GP*TP*CP*AP*TP*AP*TP*AP*TP*GP*G)-3') Chain: E
Molecule details ›
Chain: E
Length: 11 nucleotides
Theoretical weight: 3.39 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*GP*GP*TP*AP*TP*AP*TP*AP*CP*TP*G)-3') Chain: X
Molecule details ›
Chain: X
Length: 11 nucleotides
Theoretical weight: 3.39 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*AP*GP*TP*AP*TP*AP*TP*AP*CP*C)-3') Chain: Y
Molecule details ›
Chain: Y
Length: 11 nucleotides
Theoretical weight: 3.32 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P42212
Unit cell:
a: 88.966Å b: 88.966Å c: 143.445Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.249
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Plant Transcription Factors Involved in Drought and Associated Stresses.
Hrmova et al. (2021)
1 more

3 mentions without citation

Mechanism of phosphate sensing and signaling revealed by rice SPX1-PHR2 complex structure.
Zhou et al. (2021)
2 more