› 6j71

X-ray diffraction
2.92Å resolution

HuA21-scFv in complex with the extracellular domain(ECD) of HER2

Released:
DOI: 10.2210/pdb6j71/pdb
PDB entry 6j71 coloured by chain, front view.
PDB entry 6j71 coloured by chain, side view.
PDB entry 6j71 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into a matured humanized monoclonal antibody HuA21 against HER2-overexpressing cancer cells.
Wang Z, Cheng L, Guo G, Cheng B, Hu S, Zhang H, Zhu Z, Niu L
Acta Crystallogr D Struct Biol 75 554-563 (2019)
PMID: 31205018

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209121 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Receptor tyrosine-protein kinase erbB-2 Chain: A
Molecule details ›
Chain: A
Length: 618 amino acids
Theoretical weight: 68.17 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P04626 (Residues: 22-639; Coverage: 50%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains:
anti-HER2 humanized antibody HuA21 Chain: B
Molecule details ›
Chain: B
Length: 254 amino acids
Theoretical weight: 27.16 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA, NAG
Carbohydrate polymer
1 bound ligand:
Ligand BMA 1 x BMA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 146.987Å b: 78.073Å c: 125.769Å
α: 90° β: 107.47° γ: 90°
R-values:
R R work R free
0.211 0.208 0.267
Expression system: Cricetulus griseus

Quick links

Citations

1 citations in other articles

A humanized 4-1BB-targeting agonistic antibody exerts potent antitumor activity in colorectal cancer without systemic toxicity.
Cheng et al. (2022)

2 mentions without citation

CDR1 Composition Can Affect Nanobody Recombinant Expression Yields.
Orlando et al. (2021)
1 more