› 6j84

X-ray diffraction
2Å resolution

Crystal structure of TleB with hydroxyl analog

Released:
DOI: 10.2210/pdb6j84/pdb
PDB entry 6j84 coloured by chain, front view.
PDB entry 6j84 coloured by chain, side view.
PDB entry 6j84 coloured by chain, top view.
Source organism: Streptomyces blastmyceticus
Primary publication:
Molecular basis for the P450-catalyzed C-N bond formation in indolactam biosynthesis.
He F, Mori T, Morita I, Nakamura H, Alblova M, Hoshino S, Awakawa T, Abe I
Nat Chem Biol 15 1206-1213 (2019)
PMID: 31636430

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P-450 Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 47.02 KDa
Source organism: Streptomyces blastmyceticus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A077KEB8 (Residues: 1-398; Coverage: 100%)
Gene name: tleB
Sequence domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand B9U 1 x B9U
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P212121
Unit cell:
a: 39.808Å b: 84.241Å c: 112.901Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.177 0.226
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Bacterial terpenome.
Rudolf et al. (2021)
2 more