› 6j86

X-ray diffraction
2.6Å resolution

Crystal structure of HinD with NMFT

Released:
DOI: 10.2210/pdb6j86/pdb
PDB entry 6j86 coloured by chain, front view.
PDB entry 6j86 coloured by chain, side view.
PDB entry 6j86 coloured by chain, top view.
Source organism: Streptoalloteichus hindustanus
Primary publication:
Molecular basis for the P450-catalyzed C-N bond formation in indolactam biosynthesis.
He F, Mori T, Morita I, Nakamura H, Alblova M, Hoshino S, Awakawa T, Abe I
Nat Chem Biol 15 1206-1213 (2019)
PMID: 31636430

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103615 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nocardicin N-oxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 435 amino acids
Theoretical weight: 47.93 KDa
Source organism: Streptoalloteichus hindustanus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1M4Y7D5 (Residues: 1-415; Coverage: 100%)
Gene name: SAMN05444320_102263
Sequence domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand B9L 2 x B9L
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: P21
Unit cell:
a: 49.32Å b: 152.578Å c: 56.336Å
α: 90° β: 115.96° γ: 90°
R-values:
R R work R free
0.171 0.163 0.245
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Bacterial terpenome.
Rudolf et al. (2021)
2 more