› 6j94

X-ray diffraction
2.4Å resolution

Crystal structure of CYP97A3

Released:
DOI: 10.2210/pdb6j94/pdb
PDB entry 6j94 coloured by chain, front view.
PDB entry 6j94 coloured by chain, side view.
PDB entry 6j94 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for plant lutein biosynthesis from α-carotene.
Niu G, Guo Q, Wang J, Zhao S, He Y, Liu L
Proc Natl Acad Sci U S A 117 14150-14157 (2020)
PMID: 32513704

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188146 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein LUTEIN DEFICIENT 5, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 519 amino acids
Theoretical weight: 58.46 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93VK5 (Residues: 78-595; Coverage: 87%)
Gene names: At1g31800, CYP97A3, F5M6.19, LUT5
Sequence domains: Cytochrome P450

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 164.006Å b: 70.414Å c: 133.168Å
α: 90° β: 124.62° γ: 90°
R-values:
R R work R free
0.185 0.183 0.213
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Metabolic Engineering of Model Microorganisms for the Production of Xanthophyll.
Wang et al. (2023)
8 other articles

2 mentions without citation

Discovery and modification of cytochrome P450 for plant natural products biosynthesis.
Liu et al. (2020)
1 more