› 6jc5

X-ray diffraction
2.05Å resolution

Crystal structure of the blue fluorescent protein with a Leu-Leu-Gly tri-peptide chromophore derived from the purple chromoprotein of Stichodactyla haddoni

Released:
DOI: 10.2210/pdb6jc5/pdb
PDB entry 6jc5 coloured by chain, front view.
PDB entry 6jc5 coloured by chain, side view.
PDB entry 6jc5 coloured by chain, top view.
Source organism: Stichodactyla haddoni
Primary publication:
Crystal structure of the blue fluorescent protein with a Leu-Leu-Gly tri-peptide chromophore derived from the purple chromoprotein of Stichodactyla haddoni.
Chang HY, Ko TP, Chang YC, Huang KF, Lin CY, Chou HY, Chiang CY, Tsai HJ
Int J Biol Macromol 130 675-684 (2019)
PMID: 30836182

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105214 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
shBFP Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 228 amino acids
Theoretical weight: 26.08 KDa
Source organism: Stichodactyla haddoni
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A4V8H022 (Residues: 1-215; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: C2
Unit cell:
a: 103.567Å b: 98.708Å c: 197.493Å
α: 90° β: 100.09° γ: 90°
R-values:
R R work R free
0.173 0.172 0.208
Expression system: Escherichia coli

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Citations

3 citation in other articles

A novel violet fluorescent protein contains a unique oxidized tyrosine as the simplest chromophore ever reported in fluorescent proteins.
Roldán-Salgado et al. (2022)
2 more