› 6jd9

X-ray diffraction
1.58Å resolution

Proteus mirabilis lipase mutant - I118V/E130G

Released:
DOI: 10.2210/pdb6jd9/pdb
PDB entry 6jd9 coloured by chain, front view.
PDB entry 6jd9 coloured by chain, side view.
PDB entry 6jd9 coloured by chain, top view.
Source organism: Proteus mirabilis
Primary publication:
Directed evolution of a genetically encoded immobilized lipase for the efficient production of biodiesel from waste cooking oil.
Heater BS, Chan WS, Lee MM, Chan MK
Biotechnol Biofuels 12 165 (2019)
PMID: 31297153

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103914 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 31.46 KDa
Source organism: Proteus mirabilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1Z1SX23 (Residues: 2-287; Coverage: 100%)
Gene names: AM402_13560, NCTC10975_00950, lip
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: P32
Unit cell:
a: 65.31Å b: 65.31Å c: 63.591Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.14 0.139 0.163
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Chemical treatments for modification and immobilization to improve the solvent-stability of lipase.
Matsumoto et al. (2019)
2 more

2 mentions without citation

Directed evolution of a genetically encoded immobilized lipase for the efficient production of biodiesel from waste cooking oil.
Heater et al. (2019)
1 more