› 6jhb

X-ray diffraction
2.27Å resolution

Crystal structure of NADPH and 4-hydroxyphenylpyruvic acid bound AerF from Microcystis aeruginosa

Released:
DOI: 10.2210/pdb6jhb/pdb
PDB entry 6jhb coloured by chain, front view.
PDB entry 6jhb coloured by chain, side view.
PDB entry 6jhb coloured by chain, top view.
Source organism: Microcystis aeruginosa FACHB-905 = DIANCHI905
Primary publication:
Structural and functional investigation of AerF, a NADPH-dependent alkenal double bond reductase participating in the biosynthesis of Choi moiety of aeruginosin.
Qiu X, Wei Y, Zhu W, Fu J, Duan X, Jin H, Zhu P, Zhou C, Yan X
J Struct Biol 209 107415 (2020)
PMID: 31726097

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166930 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short chain dehydrogenase family protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 30.34 KDa
Source organism: Microcystis aeruginosa FACHB-905 = DIANCHI905
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand NDP 2 x NDP
Ligand ENO 1 x ENO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P4322
Unit cell:
a: 101.124Å b: 101.124Å c: 115.096Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.239 0.27
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Diversity, Biosynthesis and Bioactivity of Aeruginosins, a Family of Cyanobacteria-Derived Nonribosomal Linear Tetrapeptides.
Liu et al. (2023)
2 other articles