› 6jhe

X-ray diffraction
3.1Å resolution

Crystal Structure of Bacillus subtilis SigW domain 4 in complexed with -35 element DNA

Released:
DOI: 10.2210/pdb6jhe/pdb
PDB entry 6jhe coloured by chain, front view.
PDB entry 6jhe coloured by chain, side view.
PDB entry 6jhe coloured by chain, top view.
Source organisms:
Primary publication:
Structural analysis of the recognition of the -35 promoter element by SigW from Bacillus subtilis.
Kwon E, Devkota SR, Pathak D, Dahal P, Kim DY
PLoS One 14 e0221666 (2019)
PMID: 31461489

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120073 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
ECF RNA polymerase sigma factor SigW Chain: A
Molecule details ›
Chain: A
Length: 64 amino acids
Theoretical weight: 7.38 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: Q45585 (Residues: 126-187; Coverage: 33%)
Gene names: BSU01730, sigW, ybbL
Sequence domains: Sigma-70, region 4
DNA (5'-D(P*TP*TP*GP*AP*AP*AP*CP*CP*TP*TP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.32 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*AP*AP*AP*GP*GP*TP*TP*TP*CP*AP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.38 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P6522
Unit cell:
a: 61.62Å b: 61.62Å c: 119.971Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.25 0.248 0.29
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Extracytoplasmic Function σ Factors as Tools for Coordinating Stress Responses.
de Dios et al. (2021)
1 other article

2 mentions without citation

Structural analysis of the recognition of the -35 promoter element by SigW from Bacillus subtilis.
Kwon et al. (2019)
1 more