› 6jk3

X-ray diffraction
1.12Å resolution

Crystal structure of a mini fungal lectin, PhoSL in complex with core-fucosylated chitobiose

Released:
DOI: 10.2210/pdb6jk3/pdb
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PDB entry 6jk3 coloured by chain, front view.
PDB entry 6jk3 coloured by chain, side view.
PDB entry 6jk3 coloured by chain, top view.
Source organism: Pholiota squarrosa
Primary publication:
Structural insights into the role of N-terminal integrity in PhoSL for core-fucosylated N-glycan recognition.
Lou YC, Tu CF, Chou CC, Yeh HH, Chien CY, Sadotra S, Chen C, Yang RB, Hsu CH
Int J Biol Macromol 255 128309 (2023)
PMID: 37995778

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lectin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 40 amino acids
Theoretical weight: 4.45 KDa
Source organism: Pholiota squarrosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A3B6UEU4 (Residues: 4-43; Coverage: 93%)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: P41212
Unit cell:
a: 61.295Å b: 61.295Å c: 67.916Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 0.121 0.145
Expression system: Escherichia coli BL21(DE3)

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