› 6jkh

X-ray diffraction
3Å resolution

Function and Biology Details

Reaction catalysed: 
A 3-beta-hydroxysteroid-4-alpha-carboxylate + NAD(P)(+) = a 3-oxosteroid + CO(2) + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172386 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 27.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15738 (Residues: 31-267; Coverage: 64%)
Gene names: H105E3, NSDHL
Sequence domains: 3-beta hydroxysteroid dehydrogenase/isomerase family

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 50.027Å b: 77.366Å c: 53.993Å
α: 90° β: 96.8° γ: 90°
R-values:
R R work R free
0.176 0.173 0.238
Expression system: Escherichia coli

Quick links

Citations

2 review citations

TNBC: Potential Targeting of Multiple Receptors for a Therapeutic Breakthrough, Nanomedicine, and Immunotherapy.
Singh et al. (2021)
1 more

1 mention without citation

Structural enzymology of cholesterol biosynthesis and storage.
Long et al. (2022)