› 6jkp

X-ray diffraction
3.01Å resolution

Crystal structure of sulfoacetaldehyde reductase from Bifidobacterium kashiwanohense in complex with NAD+

Released:
DOI: 10.2210/pdb6jkp/pdb
PDB entry 6jkp coloured by chain, front view.
PDB entry 6jkp coloured by chain, side view.
PDB entry 6jkp coloured by chain, top view.
Source organism: Bifidobacterium kashiwanohense PV20-2
Primary publication:
Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense.
Zhou Y, Wei Y, Nanjaraj Urs AN, Lin L, Xu T, Hu Y, Ang EL, Zhao H, Yuchi Z, Zhang Y
Biosci Rep 39 (2019)
PMID: 31123167

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100837 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 380 amino acids
Theoretical weight: 40.89 KDa
Source organism: Bifidobacterium kashiwanohense PV20-2
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0A7I0A5 (Residues: 1-375; Coverage: 100%)
Gene name: AH68_00250
Sequence domains: Iron-containing alcohol dehydrogenase

Ligands and Environments

2 bound ligands:
Ligand NAD 4 x NAD
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 70.881Å b: 112.317Å c: 93.573Å
α: 90° β: 107.53° γ: 90°
R-values:
R R work R free
0.204 0.2 0.257
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

An extended bacterial reductive pyrimidine degradation pathway that enables nitrogen release from β-alanine.
Yin et al. (2019)
3 more

1 mention without citation

Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense.
Zhou et al. (2019)