› 6jni

X-ray diffraction
2.9Å resolution

Crystal structure of the transcriptional regulator CadR from P. putida in complex with Zinc(II) and DNA

Released:
DOI: 10.2210/pdb6jni/pdb
PDB entry 6jni coloured by chain, front view.
PDB entry 6jni coloured by chain, side view.
PDB entry 6jni coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Selective cadmium regulation mediated by a cooperative binding mechanism in CadR.
Liu X, Hu Q, Yang J, Huang S, Wei T, Chen W, He Y, Wang D, Liu Z, Wang K, Gan J, Chen H
Proc Natl Acad Sci U S A 116 20398-20403 (2019)
PMID: 31548408

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120083 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH merR-type domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 147 amino acids
Theoretical weight: 16.67 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93TP7 (Residues: 1-147; Coverage: 100%)
Gene names: BL240_26950, EFK07_13565, cadR
Sequence domains: MerR HTH family regulatory protein
DNA (25-MER) Chains: I, K, M, O
Molecule details ›
Chains: I, K, M, O
Length: 25 nucleotides
Theoretical weight: 7.73 KDa
Source organism: Pseudomonas putida
Expression system: Not provided
DNA (25-MER) Chains: J, L, N, P
Molecule details ›
Chains: J, L, N, P
Length: 25 nucleotides
Theoretical weight: 7.63 KDa
Source organism: Pseudomonas putida
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 16 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 71.09Å b: 71.329Å c: 98.797Å
α: 81.03° β: 83.52° γ: 73.85°
R-values:
R R work R free
0.214 0.212 0.254
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Molecular Evolution of Transition Metal Bioavailability at the Host-Pathogen Interface.
Antelo et al. (2021)
4 more

1 mention without citation

Selective cadmium regulation mediated by a cooperative binding mechanism in CadR.
Liu et al. (2019)